Pharmacological and Genotoxic Properties of Polyphenolic Extracts of Cedrela odorata L. and Juglans regia L. Barks in Rodents
Table 3
DNA damage according to the comet assay for the assessment of the genotoxicity of J. regia and C. odorata.
Treatments
Comet class
Total
0
1
2
3
Blood samples (4 h)
Negative control
40.5 ± 21.41a
77.0 ± 9.38a
10.7 ± 4.08a
7.2 ± 5.60a
5.2 ± 3.54a
CPA (50 mg/kg)
170.2 ± 12.31b
5.0 ± 2.28b
39.0 ± 7.01b
36.8 ± 5.52b
19.2 ± 3.12b
J. regia (100 mg/kg)
64.0 ± 52.44c
60.8 ± 25.4a
20.8 ± 6.83bc
12.0 ± 11.22c
6.4 ± 8.29a
C. odorata (100 mg/kg)
31.8 ± 8.93a
73.3 ± 6.56a
19.8 ± 3.86bc
5.5 ± 5.24a
0.3 ± 0.18c
J. regia (200 mg/kg)
58.7 ± 20.83a
65.5 ± 8.07a
15.5 ± 5.00a
13.8 ± 9.97c
5.2 ± 3.60a
C. odorata (200 mg/kg)
109.5 ± 30.48b
39.2 ± 10.94c
25.7 ± 4.67c
21.7 ± 3.26d
13.5 ± 9.28b
Blood samples (24 h)
Negative control
15.0 ± 3.79a
87.8 ± 3.37a
9.8 ± 3.54a
1.8 ± 0.75a
0.5 ± 0.83a
CPA (50 mg/kg)
96.8 ± 8.93b
29.5 ± 5.08b
47.3 ± 7.14c
20.0 ± 4.56b
3.2 ± 1.94b
J. regia (100 mg/kg)
9.4 ± 7.53a
90.8 ± 7.25a
8.6 ± 6.54a
0.4 ± 0.89a
0a
C. odorata (100 mg/kg)
12.3 ± 4.17a
88.8 ± 3.43a
10.0 ± 2.96a
1.2 ± 1.16a
0a
J. regia (200 mg/kg)
11.8 ± 4.40a
89.2 ± 3.86a
9.8 ± 3.48a
1.0 ± 0.89a
0a
C. odorata (200 mg/kg)
29.3 ± 15.88c
74.7 ± 11.96c
21.2 ± 8.95b
3.8 ± 0.86c
0.2 ± 0.40a
Mean ± standard error. Different letters between groups in each column correspond to significant differences at 4 and 24 hours. , one-way ANOVA; Fisher LSD, comparing the treatments separately at 4 and 24 hours.