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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 189891, 13 pages
http://dx.doi.org/10.1155/2015/189891
Research Article

Evaluation of an Epitypified Ophiocordyceps formosana (Cordyceps s.l.) for Its Pharmacological Potential

1Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 10617, Taiwan
2Mucho Biotechnology Inc., Taipei 10684, Taiwan
3Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan
4Department of Animal Science and Technology, National Taiwan University, Taipei 10672, Taiwan
5Center for Biotechnology, National Taiwan University, Taipei 10617, Taiwan
6Laboratory of Molecular Cell Biology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan
7Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan

Received 10 March 2015; Revised 13 July 2015; Accepted 16 July 2015

Academic Editor: Min Li

Copyright © 2015 Yen-Wen Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Degenerated primer sequences (Supporting Table 1) derived from the largest subunit of RNA polymerase II (RPB1), the second large subunit of RNA polymerase II (RPB2) and translation elongation factor-1α (EF1-α) genes were synthesized according to previous reports (30, 31, 32) and used for the molecular identifications of Cordyceps spp, (s. l.), including Ophiocordycpes formosana. The amplified 790-, 1062-, and 1068-base pair (bp) nucleotides, respectively, from our collected samples were sequenced and subjected to BLAST in the NCBI database (http://www.ncbi.nlm.nih.gov/). The closest species and strains matched, NCBI accession number, and nucleotide identify of the 3 sequences were listed (Supporting Table 2).

A list of nucleotide sequences of the 3 genes (i.e. RPB1, RPB2, and EF-1a) from 51 species/isolates was retrieved from the NCBI database following the criteria described by Sung et al or obtained from our sequenced PCR products of the O. formosana isolates (Supporting Table 3).

  1. Supplementary Material