Extra Virgin Olive Oil Polyphenols Promote Cholesterol Efflux and Improve HDL Functionality

Figure 3

EVOO-PC increases ABCA1 protein expression and enhances apoA-I-mediated cholesterol efflux. (a) [³H]-Cholesterol-loaded J774 macrophages were incubated for 12 h with various concentrations of EVOO-PC (0 to 320 μg/mL) or with cAMP (positive control) to generate ABCA1-enriched cells, which were incubated with 25 μg/mL of apo-AI for 4 h. The small upper panel shows the positive correlation between EVOO-PC concentrations and ABCA1-dependent cholesterol efflux. (b) ABCA1 protein expression after incubating J774 macrophages with increasing concentrations of EVOO-PC as determined by densitometric analyses of protein bands on PVDF membranes. Results are expressed as the means ± SEM of at least three independent experiments. , , and .

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