Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration
(a) Artocarpin-induced changes in the phosphorylation status of ERK, p38, JNK, Akt, mTOR, and S6K in HSC-1 cells, as determined by Western blotting. Whole cell extracts were prepared after artocarpin (10 μM) treatment for the times shown. The blots are representative of three independent experiments. (b) The relative protein expression levels were quantified by densitometric scanning of immunoblots after adjusting for GAPDH. Data are mean ± SEM of 3 independent experiments. , , and as compared with the time 0 hr group.