A Special Extract of Bacopa monnieri (CDRI-08) Restores Learning and Memory by Upregulating Expression of the NMDA Receptor Subunit GluN2B in the Brain of Scopolamine-Induced Amnesic Mice
Western blot (a) and semiquantitative RT-PCR (b) analysis of GluN2B expression in hippocampus. Hippocampus from 6-7 mice of each group was pooled; lysates were prepared and detected for presence of GluN2B by ECL. X-ray film was scanned and the data was expressed as relative density value (RDV) by dividing the integrated density value of GluN2B by IDV of the β-actin. The data represents mean ± SEM. C, control; BME (Bacopa monnieri extract), CDRI-08-treated; SC, scopolamine-treated; SC + BME, scopolamine-treated mice treated with CDRI-08 as in Figure 2. and #, and ## and , were considered significant. , comparison between control and other groups. #, comparison between SC and the remaining groups.