Research Article
Aqueous Extract of Agaricus blazei Murrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats
Table 1
Characteristics of primary and secondary antibodies used for immunohistochemical analysis.
| | Primary antibody | Supplier | Dilution | Secondary antibody* |
| | Anti-HuC/HuD (mouse) | Invitrogen, USA | 1 : 500 | Alexa fluor 488 (anti-mouse) | | Anti-nNOS (rabbit) | Santa Cruz Biotechnology, USA | 1 : 500 | Alexa fluor 546 (anti-rabbit) | | Anti-S-100 (rabbit) | Sigma, USA | 1 : 200 | Alexa fluor 546 (anti-rabbit) |
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The secondary antibodies were utilized with 1 : 500 dilution and supplied by Invitrogen, USA.
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