Research Article
Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds
Figure 1
A typical elution profile for the chromatography of asparaginase from P. vulgaris on DEAE-Sepharose column (15 × 1.6 cm i.d.) previously equilibrated with 20 mM Tris-HCl buffer, pH 8.0 at a flow rate of 60 mL/h and 3 mL fractions.