Research Article
Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds
Table 1
Purification scheme of asparaginase from Phaseolus vulgaris cv. Giza 6 seeds.
| Step | Total protein (mg) | Total activity (units) | S.A. (units/mg protein) | Fold purification | Recovery % |
| Crude extract | 24 | 940 | 39 | 1 | 100 | Sucrose concentration | 6 | 468 | 78 | 2 | 50 | Chromatography on DEAE-Sepharose | | | | | | 0.0 M KCl (asparaginase I) | 1.8 | 192 | 106 | 2.7 | 20 | 0.1 M KCl (asparaginase II) | 1.6 | 50 | 31 | 0.8 | 5.3 | 0.2 M KCl (asparaginase III) | 1.2 | 24 | 20 | 0.51 | 2.5 | Gel filtration on Sephacryl S-200 | | | | | | Asparaginase I | 0.15 | 127 | 846 | 21.7 | 13.5 |
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One unit of L-asparaginase activity is defined as the amount of the enzyme that liberates 1 mol of ammonia/min.
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