Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds

Table 1

Purification scheme of asparaginase from Phaseolus vulgaris cv. Giza 6 seeds.

Step	Total protein (mg)	Total activity (units)	S.A. (units/mg protein)	Fold purification	Recovery %
Crude extract	24	940	39	1	100
Sucrose concentration	6	468	78	2	50
Chromatography on DEAE-Sepharose
0.0 M KCl (asparaginase I)	1.8	192	106	2.7	20
0.1 M KCl (asparaginase II)	1.6	50	31	0.8	5.3
0.2 M KCl (asparaginase III)	1.2	24	20	0.51	2.5
Gel filtration on Sephacryl S-200
Asparaginase I	0.15	127	846	21.7	13.5

One unit of L-asparaginase activity is defined as the amount of the enzyme that liberates 1 mol of ammonia/min.