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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 316017, 8 pages
http://dx.doi.org/10.1155/2015/316017
Research Article

Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa

1Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil
2Faculdade São Francisco de Barreiras, BR 135 Km 01 2341, 47800-970 Barreiras, BA, Brazil
3Faculdade de Ciências Integradas do Pontal, Universidade Federal de Uberlândia, Rua 20 1600, 38304-402 Ituiutaba, MG, Brazil
4Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida Pará 1720, 38400-902 Uberlândia, MG, Brazil

Received 15 August 2014; Revised 23 December 2014; Accepted 24 December 2014

Academic Editor: Wenchuan Lin

Copyright © 2015 Izabela Barbosa Moraes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins. Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM. Despite its popularity, there is little information about its effect on hepatic tissue. Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV). Histology was determined by fixing the livers, processing, and staining with HE. Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA). Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry. In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased. AEV treatment restored all parameters to normal levels. The oxidative stress analysis of hepatic mitochondria fraction showed similar results. Lower polyploid cell populations were found in the diabetic rat livers, even after glibenclamide treatment. Thus, AEV treatment efficiently reduced hepatic oxidative stress caused by STZ-induced diabetes and produced no morphological changes in the histological analysis.