[Retracted] Sagunja-Tang Improves Lipid Related Disease in a Postmenopausal Rat Model and HepG2 Cells

Figure 5

Effects of SGJT on cholesterol synthesis in HepG2 cells. HepG2 cells were treated with 30 μM simvastatin or SGJT (250, 500 μg/mL) with 20 mg/mL MβCD for 8 h in 0.2% BSA-DMEM. Cell lysates were then harvested by RIPA buffer and subjected to HMG-CoA reductase activity and inhibition assays (a) and Western blotting analysis for SREBP2, HMGCR, and LDLR protein expression (b). Quantified data of protein levels indicated in lower panel. The values of density of proteins were all justified with β-actin. The relative density ratios of untreated cells were set at a value of 1.0. Values not sharing a common alphabet as superscripts are significantly different from each other at the level of (ANOVA followed by Duncan’s test). Similar results were obtained in three separate experiments.

(a)

(b)