Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

Figure 4

Apoptosis induced by rhein. (a) A representative result of flow cytometry of HL-7702 cells stained with Annexin V/PI, after treatment with rhein at concentration of 25 μM, 50 μM, and 100 μM for 12 h (Annexin V−/PI− represents viable cells, Annexin V+/PI− represents early apoptotic cells, and Annexin V+/PI+ represents late apoptosis or necrotic cells). (b) The experiment was repeated three times and the percentage of early apoptotic cells (mean ± SD) for each treatment group is shown ( versus control).

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