An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1–7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils

Figure 3

The effect of essential oils on GT1–7 cells exposed with various cytotoxins. GT1–7 cells were treated with 20 μM H₂O₂ (a), 100 μM Al (b), or 25 μM Zn (c) without (aroma (—) in graph) or with 25 ppm of various essential oils. After 24 h, the viability was analyzed using the WST-1 method. The white bar shows the viability of GT1–7 cells treated with essential oil alone. Data are presented as the mean ± SEM (). (d) The dose dependency of the effects of essential oils on GT1–7 cell cytotoxicity. 10–25 ppm of essential oils was treated with 20 μM H₂O₂. After 24 h, the viability was analyzed using the WST-1 method. Data are presented as the mean ± SEM (). , versus the aroma (—) group. The abbreviated names of the essential oils used in the figure are shown in Table 1.

(a) With H₂O₂

(b) With Al-maltol

(c) With Zn

(d) Dose response