ACE Reduces Metabolic Abnormalities in a High-Fat Diet Mouse Model

Figure 1

Fingerprint analysis of ACE using high-performance thin-layer chromatography (HP-TLC). ACE and its two major components were analyzed using HP-TLC and compared with reference compounds. Scopoletin (SCL, 0.1 μg/μL (a)), Artemisia iwayomogi (AI, 10 μg/μL (a)), curcumin (CUR, 0.1 μg/μL (b)), Curcuma longa radix (CL, 10 μg/μL (b)), and ACE (10 μg/μL ((a) and (b))) were applied to prewashed silica gel 60 F254 TLC plates and then separated in the mobile phase (chloroform : ethyl acetate : methanol : water = 17 : 46 : 25 : 12). The migrated components were visualized under UV light at 254 nm (left) or 366 nm (right).

(a)

(b)