Research Article

Naringin Attenuates Autophagic Stress and Neuroinflammation in Kainic Acid-Treated Hippocampus In Vivo

Figure 4

Anti-inflammatory effects of naringin in the KA-treated hippocampus. Similar to experiments for autophagic stress, mice received treatment with naringin. Representative pictures of immunoperoxidase staining for Iba1 in the hippocampus (a). Morphologic changes of KA-induced microglia activation are found at 1 day after KA treatment in the hippocampus compared with CON; however, treatment with naringin decreases microglia activation triggered by KA excitotoxicity. Scale bar: 200 μm and 50 μm, respectively. Consistent with naringin treatment-induced deactivation of microglia, treatment with naringin shows reduction of TNFα expression in Iba1-positive microglial cells of the hippocampus (b). Scale bar: 10 μm. Western blot analysis shows that treatment with naringin significantly reduces the KA-increased levels of TNFα compared to KA alone (c). Quantitative data are calculated from the density of TNFα bands normalized to the density of β-actin bands for each sample. , significantly different from CON and KA alone, respectively (one-way ANOVA and Tukey post hoc test, for each group).
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