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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 369631, 6 pages
http://dx.doi.org/10.1155/2015/369631
Research Article

Effects of Cordyceps sinensis on the Expressions of NF-κB and TGF-β1 in Myocardium of Diabetic Rats

1Department of Endocrinology, The Fifth Central Hospital of Tianjin, Tianjin 300450, China
2Community Health Service Center of Hu Jiayuan Street, Binhai New District, Tianjin 300454, China
3Graduate School of Tianjin Medical University, Tianjin 300070, China
4Department of Endocrinology, General Hospital of Tianjin Medical University, Tianjin 300052, China

Received 23 March 2015; Revised 26 July 2015; Accepted 2 August 2015

Academic Editor: Ken Yasukawa

Copyright © 2015 You-you Gu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To investigate the effect of Cordyceps sinensis (CS) on the expressions of NF-κB and TGF-β1 in myocardium of streptozotocin-induced diabetic rats. Methods. A total of 53 healthy male SD rats, mice age of 8 weeks and weight of 220 ± 20 g, were randomly divided into five groups by randomized block design: normal control group (), diabetic group (), low dose of CS group (; CS 0.6 g·kg−1·d−1), middle dose of CS group (; CS 2.5 g·kg−1·d−1), and high dose of CS group (; CS 5 g·kg−1·d−1). The diabetic models with tail intravenous injection by streptozotocin (45 mg·kg−1). Diabetic rats were sacrificed after 8 weeks; the expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle were determined by using immunohistochemistry staining and reverse transcription polymerase chain reaction (RT-PCR) method. The data were analyzed using one factor analysis of variance. Result. The expressions of NF-κB and TGF-β1 proteins and mRNA in the cardiac muscle of diabetic rats were significantly raised (), which could be decreased by CS (). Conclusions. The changes on the expressions of NF-κB and TGF-β1 in myocardium may be involved in the occurrence of diabetic cardiomyopathy (DC). CS may play its role on myocardial protection by regulating the expressions of NF-κB and TGF-β1 in myocardium.