Research Article

Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

Figure 3

EVA abrogates VEGF induced tube formation in HUVECs. (a) Matrigel (250 μL) was added to 24-well plates and allowed to solidify for 1 h at 37°C. HUVECs (1 105 cells/well) were treated with 10 and 20 μg/mL of EVA in the presence or absence of VEGF (50 ng/mL) and incubated at 37°C. After 18 h, randomly chosen fields were photographed under an Axiovert S 100 light microscope. (b) Bar graph represents the quantification of tube formation of untreated control or VEGF-treated control and EVA-treated groups. Data are presented as means ± SD. versus VEGF-treated group.