Research Article

Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

Figure 7

Akt, Erk inhibitors, and EVA attenuate tube formation enhanced by VEGF in HUVECs. (a) Matrigel (250 μL) was added to 24-well plates and EVA (20 μg/mL), LY294002 (Akt inhibitor, 20 μM), and PD98059 (Erk inhibitor, 50 μM) were treated to HUVECs (1 105 cells/well) in the presence of VEGF (50 ng/mL) and incubated at 37°C. After 18 h, randomly chosen fields were photographed under an Axiovert S 100 light microscope. (b) Bar graph represents the quantification of tube formation of each group. Data are presented as means ± SD. versus VEGF-treated group.