Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Tab 1 / Research Article
Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A2A Receptors Table 1 Adenosine receptor A2A binding assay parameters.
Adenosine A2A Source Human recombinant HEK-293 cells Ligand 0.05 M [3 ] CGS-21680 Vehicle 1% DMSO Incubation time/temp. 90 minutes at 25°C Incubation buffer 50 mM Tris-HCl, pH 7.4, 10 mM MgCl2 , 1 mM EDTA, 2 U/mL adenosine Deaminase Nonspecific ligand 50 M NECA (5-N-ethylcarboxamide adenosine) KD 0.064 M 7 pmole/mg protein Specific binding 85% Quantitation method Radioligand binding Significance criteria
≥50% of max stimulation Reference CGS-21680