Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Tab 1

Research Article

Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A2A Receptors

Table 1

Adenosine receptor A2A binding assay parameters.

Adenosine A2A

SourceHuman recombinant HEK-293 cells
Ligand0.05 M [3] CGS-21680
Vehicle1% DMSO
Incubation time/temp. 90 minutes at 25°C
Incubation buffer50 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM EDTA, 2 U/mL adenosine Deaminase
Nonspecific ligand50 M NECA (5-N-ethylcarboxamide adenosine)
KD0.064 M
7 pmole/mg protein
Specific binding85%
Quantitation methodRadioligand binding
Significance criteria ≥50% of max stimulation

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