Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A_2A Receptors

Table 3

Adenosine receptor A₁ functional assay parameter.

Adenosine A1
Source	Wistar rat vas deferens
Control	0.3 M CHA (N6-cyclohexyladenosine)
Vehicle	0.10% DMSO
Incubation time/temp.	5 minutes at 32°C
Incubation buffer	KREBS pH 7.4
Quantitation method	Isometric (gram changes)
Significant criteria for agonist	≥50% reduction of neurogenic twitch relative to 0.3 M CHA response
Significant criteria for antagonist	≥50% inhibition of 0.3 M CHA-induced relaxation