Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice

Figure 1

Qualitative assessment of L. plantarum K21 for hydrolyzing bile salt and inhibiting lipid accumulation in 3T3-L1 preadipocytes. (a) Demonstration of the ability of K21 to hydrolyze bile salt. The K21 strain was inoculated on an MRS agar plate (NC, negative control) or an MRS agar plate containing 0.5% TDCA, as indicated. The plates were incubated at 37°C under anaerobic conditions for three days, and the colonial morphology was observed. In the assays shown, precipitation in the agar is indicative of bacterial ability for hydrolyzing TDCA. (b) The inhibitory effects of K21 on lipid accumulation in 3T3-L1 preadipocytes. 3T3-L1 cells were differentiated with differentiation medium (DM) in the absence or presence of heat-killed K21 (~10⁷ CFU/mL) as described in Materials and Methods. Intracellular lipids were stained with Oil Red-O. Non, undifferentiated cells. The results were confirmed with three independent experiments.

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