Berberine Regulated Lipid Metabolism in the Presence of C75, Compound C, and TOFA in Breast Cancer Cell Line MCF-7

Figure 3

ROS level of MCF-7 cells in presence of CC and TOFA. ROS level of MCF-7 cells was assayed by H₂DCF-DA/Hoechst 33342 after pretreatment of CC (4 μg/mL) and TOFA (10 μg/mL) for 3 hours and treatment of berberine (25 μM) for 24 hours. ROS generation was presented as percentage of control, and all significant difference was calculated by Student’s test and presented as and , value and .