DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus)

Table 2

Identification results of JBS crude drug samples.

Samples	Commodity name	Producing area	Macroscopical inspection	PCR-RFLP	Diagnostic PCR	DNA barcoding	Sequence similarity
D1	Xiao Baihua She	Guangxi	B. multicinctus	+	+	B. multicinctus	100%
D2	Xiao Baihua She	Guangxi	B. multicinctus	+	+	B. multicinctus	100%
D3	Da Baihua She	Guangxi	B. multicinctus	+	+	B. multicinctus	100%
D4	Xiao Baihua She	Hunan	B. multicinctus	+	+	B. multicinctus	99.85%
D5	Jinqian Baihua She	Guangdong	B. multicinctus	+	+	B. multicinctus	100%
D6	Baihua She	Guangdong	Di. rufozonatum	−	−	Di. rufozonatum	99.85%
D7	Baihua She	Unknown	Di. rufozonatum	−	−	Di. rufozonatum	99.69%
D8	Baihua She	Unknown	S. annularis	−	−	S. annularis	99.24%
D9	Baihua She	Unknown	En. chinensis	−	−	En. chinensis	98%
D10	Baihua She	Unknown	Di. rufozonatum	−	−	Di. rufozonatum	99.54%
D11	Xiao Baihua She	Guangxi	Colubridae sp.	−	−	Di. rufozonatum	99.24%

+: Jinqian Baihua She; −: adulterant.