The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells

Figure 1

Effects of YC on Nur77 mRNA and protein expression in MLTC-1 cells. (a and b) MLTC-1 cells were treated with YC (1 mg/mL) for 0 h, 1 h, 2 h, 4 h, and 8 h, respectively. (c and d) MLTC-1 cells were treated with 0, 0.01, 0.1, and 1 mg/mL YC and 0.1 U/mL hCG for 2 h. mRNA and protein expression levels were assessed by real-time PCR and Western blot, respectively. Data are a percentage of the control set to 100%. The values are mean ± SD from three independent experiments. Compared with the control, and .

(a)

(b)

(c)

(d)