Extract of Allium tuberosum Rottler ex Spreng Promoted the Hair Growth through Regulating the Expression of IGF-1

Figure 4

The n-butanol soluble fraction was further separated using silica gel chromatography. (a) The n-butanol extract was dipped onto the TLC plate and developed. To isolate the four different subfractions (n1, n2, n3, and n4), the n-butanol soluble fraction was loaded onto the silica gel column and eluted to 400 vials (1 mL per vial). (b) The representative TLC figure for each fraction (n1, n2, n3, and n4) is shown. Elutes in each of the every five vials were tested via TLC. Elutes that showed the same TLC result were mixed and then dried. Cont. refers to the column that was loaded with the n-butanol extract.

(a)

(b)