Effect of Electroacupuncture on Cell Apoptosis and ERK Signal Pathway in the Hippocampus of Adult Rats with Cerebral Ischemia-Reperfusion

Figure 3

(a) Bar graph showing the apoptotic index of nerve cells. At the end of the experiment, cerebral tissues from each group () were processed for TUNEL assay. Nuclei of all cells were visualized by DAPI staining, and apoptotic cells stained brown were detected by microscope. Apoptotic cells were counted at 4 arbitrarily selected microscopic fields at a magnification of ×200. Apoptotic rate was expressed as the ratio of brown-stained cells to the DAPI-stained total cells. Data is expressed as mean ± SE (error bars). At the same time point, versus I/R group, , ; versus PD group, ^▲, ^▲▲. (b) Apoptosis of neural cells observed under a light microscope (TUNEL, ×200).

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