Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway

Figure 3

The effect of SCU on the protein level of PKG-I, VASP, and p-VASP in HR HBMECs. (a) Representative immunoblot and quantification of PKG-I, p-VASP, and VASP protein expression in HR HBMECs with SCU treatment. (b) The ratio of p-VASP and VASP compared with control in HR HBMECs with SCU treatment. Protein ratio of control was calculated by determining band integrated intensity as ratio of control. (c) The ratio of p-VASP compared with VASP in HR HBMECs with SCU treatment. (d) The ratio of PKG-I compared with control in HR HBMECs with SCU treatment. Protein ratio of control was calculated by determining band integrated intensity as ratio of control. Control group: cells were treated with vehicle control (NS) under normal culture condition for 26 h. Model group: cells were incubated under normal culture condition for 2 h and then given HR treatment (hypoxia 12/reoxygenation 12 hours). SCU groups: cells were incubated with SCU for 2 h prior to HR injury. One-way ANOVA followed by SNK test, , , compared to model of HR group, , , compared to control group. Data are means ± SEM; independent experiments with independent culture.

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