Scutellarin Reduces Endothelium Dysfunction through the PKG-I Pathway

Figure 4

The effect of scutellarin (SCU) on mRNA expression of PKG and cell viability in HR HBMECs. (a) Representative electrophotogram and quantification of PKG-Iα mRNA expression in HR HBMECs. (b) The mRNA ratio of PKG-Iα in HR HBMECs. Ratio of control was calculated by determining band integrated intensity as ratio of control. (c) SCU increases cell viability in hypoxia reoxygenation- (HR-) treated human brain microvascular endothelial cells (HBMECs). Cell viability was examined using MTT assay and expressed as ratio of control. Control group: cells were treated with vehicle control (NS) under normal culture condition for 26 h. Model group: cells were incubated under normal culture condition for 2 h and then given HR treatment (hypoxia 12/reoxygenation 12 hours). One-way ANOVA followed by SNK test, , , compared to model of HR group, , , compared to control group. Data are means ± SEM; independent experiments with independent culture.

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