Suppression of Propionibacterium acnes-Induced Dermatitis by a Traditional Japanese Medicine, Jumihaidokuto, Modifying Macrophage Functions

Figure 6

Jumihaidokuto had no effect on chemokine concentrations in inflamed ears. The pinnae were collected at 2 and 24 h after injection of P. acnes. Each sample was homogenized in cold PBS supplemented with proteinase inhibitors and centrifuged. CINC-1 (a) and MCP-1 (b) concentrations in the supernatants were determined by ELISA. Jumihaidokuto (JHT, 0.5 g/kg), prednisolone (PDN, 10 mg/kg), or distilled water (DW) was administered orally 1 h before and 6 h after bacterial injection. (2 h), 4-5 (24 h). significant versus saline + DW group (Student’s -test), and 0.01, respectively, and significant versus P. acnes + DW group (Dunnett’s test).

(a)

(b)