Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Tab 1

Research Article

Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography

Table 1

Yields of the investigated components obtained through the heat-reflux extraction (HRE) method with various solvents (one extraction cycle) and the pure SC–CO2 extraction method.

Methods/solventsExtraction yield (mg/g)a
OAUA

HREb
 Nonpolar
  Chloroform 0.582 ± 0.0232.580 ± 0.105
  -Hexane Not detectedNot detected
 polar
  0% Ethanol (water)Not detectedNot detected
  10% EthanolNot detectedNot detected
  20% EthanolNot detectedNot detected
  30% EthanolNot detectedNot detected
  40% EthanolNot detectedNot detected
  50% EthanolNot detectedNot detected
  60% Ethanol0.544 ± 0.0211.171 ± 0.045
  70% Ethanol0.661 ± 0.0251.674 ± 0.060
  80% Ethanol0.650 ± 0.0242.283 ± 0.082
  90% Ethanol0.629 ± 0.0222.601 ± 0.093
  95% Ethanol0.623 ± 0.0252.340 ± 0.092
  99.5% Ethanol0.599 ± 0.0242.211 ± 0.081
Pure SC−Not detectedNot detected

Values are mean ± SD of six replications and are calculated on plant dry weight basis (HD3).
bThe experimental conditions are described in the experimental section.
cPure SC−CO2 conditions: extraction pressure at 10.4–30.0 MPa, extraction temperature at 40–70°C, a static extraction time of 30 min, a dynamic extraction time of 10–180 min, mean particle size at 0.096–0.925 mm, and CO2 flow rate at 0.6–2.5 mL/min (STP).

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