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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 462864, 8 pages
http://dx.doi.org/10.1155/2015/462864
Research Article

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture of Cordyceps gracilis (Grev.) Durieu & Mont.

1Department of Plant Pathology, CSK, Himachal Pradesh Agriculture University, Palampur 176 062, India
2Centre for Environment Science and Technology, School of Environmental and Earth Sciences, Central University of Punjab, Bathinda 151 001, India
3Department of Botany, Punjabi University, Patiala, Punjab 147 002, India

Received 29 November 2014; Revised 18 February 2015; Accepted 27 February 2015

Academic Editor: Zheng L. Jiang

Copyright © 2015 Sapan Kumar Sharma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. Y. Kobayasi, “Keys to the taxa of the genera Cordyceps and Torrubiella,” Transactions of the Mycological Society of Japan, vol. 23, pp. 329–364, 1982. View at Google Scholar
  2. Ø. Stensrud, N. L. Hywel-Jones, and T. Schumacher, “Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly,” Mycological Research, vol. 109, no. 1, pp. 41–56, 2005. View at Publisher · View at Google Scholar · View at Scopus
  3. P. M. Kirk, P. Cannon, J. C. David, and J. A. Stalpers, Ainsworth and Bisby's Dictionary of the Fungi, CAB International, Wallingford, UK, 10th edition, 2008.
  4. D.-B. Ji, J. Ye, C.-L. Li, Y.-H. Wang, J. Zhao, and S.-Q. Cai, “Antiaging effect of Cordyceps sinensis extract,” Phytotherapy Research, vol. 23, no. 1, pp. 116–122, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. T. Kiho, A. Yamane, J. Hui, S. Usui, and S. Ukai, “Polysaccharides in fungi. XXXVI.1 Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver,” Biological and Pharmaceutical Bulletin, vol. 19, no. 2, pp. 294–296, 1996. View at Publisher · View at Google Scholar · View at Scopus
  6. Y.-J. Chen, M.-S. Shiao, S.-S. Lee, and S.-Y. Wang, “Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U937 cells,” Life Sciences, vol. 60, no. 25, pp. 2349–2359, 1997. View at Publisher · View at Google Scholar · View at Scopus
  7. Y. B. Ji, “Pharmacological actions and applications of anticancer traditional Chinese medicines (150. Cordycepssinensis (Berk) Sacc),” Heilongjiang Ke-Xue-Ji-Shu Chu-Ban-She, pp. 494–501, 1999 (Chinese). View at Google Scholar
  8. T. Mizuno, “Medicinal effects and utilization of Cordyceps (Fr.) link (Ascomycetes) and Isaria Fr. (Mitosporic fungi) Chinese caterpillar fungi, ‘Tochukaso’ (Review),” International Journal of Medicinal Mushrooms, vol. 1, no. 3, pp. 251–261, 1999. View at Publisher · View at Google Scholar
  9. S. W. Kim, H. J. Hwang, C. P. Xu, Y. S. Na, S. K. Song, and J. W. Yun, “Influence of nutritional conditions on the mycelial growth and exopolysaccharide production in Paecilomyces sinclairii,” Letters in Applied Microbiology, vol. 34, no. 6, pp. 389–393, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. C. S. Zhao, W. T. Yin, J. Y. Wang et al., “CordyMax Cs-4 improves glucose metabolism and increases insulin sensitivity in normal rats,” Journal of Alternative and Complementary Medicine, vol. 8, no. 3, pp. 309–314, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. J. E. Smith, N. J. Rowan, and R. Sullivan, “Medicinal mushrooms: a rapidly developing area of biotechnology for cancer therapy and other bioactivities,” Biotechnology Letters, vol. 24, no. 22, pp. 1839–1845, 2002. View at Publisher · View at Google Scholar · View at Scopus
  12. X. Ma, D. K. Qiu, J. Xu, J. Q. Li, and M. D. Zeng, “Effect of Cordyceps polysaccharide-liposome on transforming growth factor b-1 in the experimental liver fibrotic rats,” Chinese Journal of Gastroenterology, vol. 4, pp. 205–206, 1999. View at Google Scholar
  13. C. Lu, Preventive activity of Cordyceps polysaccharides on nonalcoholic steatohepatitis and their partial mechanismsof action [M.S. thesis], Anhui Medical University, Anhui, China, 2005.
  14. E. J. Buenz, B. A. Bauer, T. W. Osmundson, and T. J. Motley, “The traditional Chinese medicine Cordyceps sinensis and its effects on apoptotic homeostasis,” Journal of Ethnopharmacology, vol. 96, no. 1-2, pp. 19–29, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. F.-H. Li, P. Liu, W.-G. Xiong, and G.-F. Xu, “Effects of Corydyceps polysaccharide on liver fibrosis induced by DMN in rats,” China Journal of Chinese Materia Medica, vol. 31, no. 23, pp. 1968–1971, 2006. View at Google Scholar · View at Scopus
  16. J. Chen, W. Zhang, T. Lu, J. Li, Y. Zheng, and L. Kong, “Morphological and genetic characterization of a cultivated Cordyceps sinensis fungus and its polysaccharide component possessing antioxidant property in H22 tumor-bearing mice,” Life Sciences, vol. 78, no. 23, pp. 2742–2748, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. J. L. Liu and Y. Fei, “Enhancement of Cordyceps taii polysaccharide and Cordyceps pruinosa polysaccharide on cellular immune function in vitro,” Journal of Immunology, vol. 17, no. 3, pp. 189–191, 2001. View at Google Scholar
  18. J. Yang, W. Zhang, P. Shi, J. Chen, X. Han, and Y. Wang, “Effects of exopolysaccharide fraction (EPSF) from a cultivated Cordyceps sinensis fungus on c-Myc, c-Fos, and VEGF expression in B16 melanoma-bearing mice,” Pathology Research and Practice, vol. 201, no. 11, pp. 745–750, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. H. O. Kim and J. W. Yun, “A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures,” Journal of Applied Microbiology, vol. 99, no. 4, pp. 728–738, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. C. Hobbs, Medicinal Mushrooms: An Exploration of Tradition, Healing, and Culture, Botanica Press, Santa Cruz, Calif, USA, 1995.
  21. R. Yu, L. Song, Y. Zhao et al., “Isolation and biological properties of polysaccharide CPS-1 from cultured Cordyceps militaris,” Fitoterapia, vol. 75, no. 5, pp. 465–472, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. T.-H. Hsu, L.-H. Shiao, C. Hsieh, and D.-M. Chang, “A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom Dong Chong Xia Cao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis,” Food Chemistry, vol. 78, no. 4, pp. 463–469, 2002. View at Publisher · View at Google Scholar · View at Scopus
  23. M.-F. Moradali, H. Mostafavi, S. Ghods, and G.-A. Hedjaroude, “Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi),” International Immunopharmacology, vol. 7, no. 6, pp. 701–724, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. J.-H. Koh, J.-M. Kim, U.-J. Chang, and H.-J. Suh, “Hypocholesterolemic effect of hot-water extract from mycelia of Cordyceps sinensis,” Biological and Pharmaceutical Bulletin, vol. 26, no. 1, pp. 84–87, 2003. View at Publisher · View at Google Scholar · View at Scopus
  25. S. P. Li, K. J. Zhao, Z. N. Ji et al., “A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury,” Life Sciences, vol. 73, no. 19, pp. 2503–2513, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. B. M. Huang, S. Y. Ju, C. S. Wu, W. J. Chuang, C. C. Sheu, and S. F. Leu, “Cordyceps sinensis and its fractions stimulate MA-10 mouse leydig tumor cell steroidogenesis,” Journal of Andrology, vol. 22, no. 5, pp. 831–837, 2001. View at Google Scholar · View at Scopus
  27. F. Meng, X. Liu, L. Jia, Z. Song, P. Deng, and K. Fan, “Optimization for the production of exopolysaccharides from Morchella esculenta SO-02 in submerged culture and its antioxidant activities in vitro,” Carbohydrate Polymers, vol. 79, no. 3, pp. 700–704, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. X.-X. Lin, Q.-M. Xie, W.-H. Shen, and Y. Chen, “Effects of fermented Cordyceps powder on pulmonary function in sensitized guinea pigs and airway inflammation in sensitized rats,” Zhongguo Zhong Yao Za Zhi, vol. 26, no. 9, pp. 622–625, 2001. View at Google Scholar · View at Scopus
  29. R. M. Yu, Y. Yin, W. Yang et al., “Structural elucidation and biological activity of a novel polysaccharide by alkaline extraction from cultured Cordyceps militaris,” Carbohydrate Polymers, vol. 75, no. 1, pp. 166–171, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. F. Shahidi and P. K. J. P. D. Wanasundara, “Phenolic antioxidants,” Critical rReviews in Food Science and Nutrition, vol. 32, no. 1, pp. 67–103, 1992. View at Publisher · View at Google Scholar · View at Scopus
  31. N. Abdullah, S. M. Ismail, N. Aminudin, A. S. Shuib, and B. F. Lau, “Evaluation of selected culinary-medicinal mushrooms for antioxidant and ACE inhibitory activities,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 464238, 12 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. T. H. Bang, H. Suhara, K. Doi et al., “Wild mushrooms in Nepal: some potential candidates as antioxidant and ACE-inhibition sources,” Evidence-Based Complementary and Alternative Medicine, vol. 2014, Article ID 195305, 11 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  33. H.-C. Huang and Y.-C. Liu, “Enhancement of polysaccharide production by optimization of culture conditions in shake flask submerged cultivation of Grifola umbellata,” Journal of the Chinese Institute of Chemical Engineers, vol. 39, no. 4, pp. 307–311, 2008. View at Publisher · View at Google Scholar · View at Scopus
  34. C. P. Pokhrel and S. Ohga, “Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes,” Food Chemistry, vol. 105, no. 2, pp. 641–646, 2007. View at Publisher · View at Google Scholar · View at Scopus
  35. D.-H. Kim, B.-K. Yang, S.-C. Jeong et al., “Production of a hypoglycemic, extracellular polysaccharide from the submerged culture of the mushroom, Phellinus linteus,” Biotechnology Letters, vol. 23, no. 7, pp. 513–517, 2001. View at Publisher · View at Google Scholar · View at Scopus
  36. M. Papagianni, “Fungal morphology and metabolite production in submerged mycelial processes,” Biotechnology Advances, vol. 22, no. 3, pp. 189–259, 2004. View at Publisher · View at Google Scholar · View at Scopus
  37. X. U. Qinqin, L. I. U. Zhenhua, S. U. N. Yisheng, D. I. N. G. Zhongjie, L. Ü. Longxian, and L. I. Yongquan, “Optimization for production of intracellular polysaccharide from Cordyceps ophioglossoides L2 in submerged culture and its antioxidant activities in vitro,” Chinese Journal of Chemical Engineering, vol. 20, no. 2, pp. 294–301, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. Q. Xu, L. Lü, S. Chen, G. Zheng, J. Zheng, and Y. Li, “Isolation of Cordyceps ophioglossoides L2 from fruit body and optimization of fermentation conditions for its mycelial growth,” Chinese Journal of Chemical Engineering, vol. 17, no. 2, pp. 278–285, 2009. View at Publisher · View at Google Scholar · View at Scopus
  39. Q.-H. Fang and J.-J. Zhong, “Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites—ganoderic acid and polysaccharide,” Biochemical Engineering Journal, vol. 10, no. 1, pp. 61–65, 2002. View at Publisher · View at Google Scholar · View at Scopus
  40. M.-Y. Lung and Y.-C. Chang, “In vitro antioxidant properties of polysaccharides from Armillaria mellea in batch fermentation,” African Journal of Biotechnology, vol. 10, no. 36, pp. 7048–7057, 2011. View at Google Scholar · View at Scopus
  41. N. S. Atri, S. K. Sharma, R. Joshi, and A. Gulati, “Nutritional and neutraceutical composition of five wild culinary-medicinal species of genus Pleurotus (Higher Basidiomycetes) from Northwest India,” International Journal of Medicinal Mushrooms, vol. 15, no. 1, pp. 49–56, 2013. View at Publisher · View at Google Scholar · View at Scopus
  42. E. Vamanu, “Biological activities of the polysaccharides produced in submerged culture of two edible Pleurotus ostreatus mushrooms,” Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 565974, 8 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  43. P. Li, L. Huo, W. Su et al., “Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica,” Journal of the Serbian Chemical Society, vol. 76, no. 5, pp. 709–717, 2011. View at Publisher · View at Google Scholar · View at Scopus
  44. C. M. Papuc, M. Crivineanu, G. Goran, V. Nicorescu, and N. Durdun, “Free radicals scavenging and antioxidant activity of European mistletoe (Viscum album) and European birthwort (Aristolochia clematitis),” Revista de Chimie, vol. 61, no. 7, pp. 619–622, 2010. View at Google Scholar · View at Scopus
  45. V. O. Oyetayo, C.-H. Dong, Y.-J. Yao, and T. Jackson, “Antioxidant and antimicrobial properties of aqueous extract from Dictyophora indusiata,” The Open Mycology Journal, vol. 3, no. 1, pp. 20–26, 2009. View at Publisher · View at Google Scholar
  46. C. Hsieh, M.-J. Tsai, T.-H. Hsu, D.-M. Chang, and C.-T. Lo, “Medium optimization for polysaccharide production of Cordyceps sinensis,” Applied Biochemistry and Biotechnology, vol. 120, no. 2, pp. 145–157, 2005. View at Publisher · View at Google Scholar · View at Scopus
  47. C.-H. Dong and Y.-J. Yao, “Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture,” Journal of Applied Microbiology, vol. 99, no. 3, pp. 483–492, 2005. View at Publisher · View at Google Scholar · View at Scopus
  48. Y. S. Kim, K. S. Park, H. S. Park, and S. W. Kim, “Compositional sugar analysis of antitumor polysaccharides by high performance liquid chromatography and gas chromatography,” Archives of Pharmacal Research, vol. 17, no. 5, pp. 337–342, 1994. View at Publisher · View at Google Scholar · View at Scopus
  49. Y. Kwan-Won, H.-J. Suh, S. H. Bae, C. S. Lee, S. H. Kim, and C.-S. Yoon, “Chemical properties and physiological activities of stromata of Cordyceps militaris,” Journal of Microbiology and Biotechnology, vol. 11, no. 2, pp. 266–274, 2001. View at Google Scholar · View at Scopus
  50. H. Yan, D. Zhu, D. Xu, J. Wu, and X. Bian, “A study on Cordyceps militaris polysaccharide purification, composition and activity analysis,” African Journal of Biotechnology, vol. 7, no. 22, pp. 4004–4009, 2008. View at Google Scholar · View at Scopus
  51. Y.-X. Gu, Y.-W. Song, L.-Q. Fan, and Q.-S. Yuan, “Antioxidant activity of natural and cultured Cordyceps sp.,” Zhongguo Zhong Yao Za Zhi, vol. 32, no. 11, pp. 1028–1031, 2007. View at Google Scholar · View at Scopus
  52. X. Yuan, H. Sun, Y. Liu et al., “Anti-cancer activity comparisons of aqueous extracts from Inonotus obliquus, Cordyceps militaris and Uncaria tomentosain vitro and in vivo,” Journal of Pharmacognosy and Phytochemistry, vol. 2, no. 6, pp. 19–25, 2014. View at Google Scholar
  53. P. H. Leung, S. Zhao, K. P. Ho, and J. Y. Wu, “Chemical properties and antioxidant activity of exopolysaccharides from mycelial culture of Cordyceps sinensis fungus Cs-HK1,” Food Chemistry, vol. 114, no. 4, pp. 1251–1256, 2009. View at Publisher · View at Google Scholar · View at Scopus
  54. C.-H. Dong, T. Yang, and T. Lian, “A comparative study of the antimicrobial, antioxidant, and cytotoxic activities of methanol extracts from fruit bodies and fermented mycelia of caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes),” International Journal of Medicinal Mushrooms, vol. 16, no. 5, pp. 485–495, 2014. View at Publisher · View at Google Scholar
  55. J. A. Vinson, Y. Hao, X. Su, and L. Zubik, “Phenol antioxidant quantity and quality in foods: vegetables,” Journal of Agricultural and Food Chemistry, vol. 46, no. 9, pp. 3630–3634, 1998. View at Publisher · View at Google Scholar · View at Scopus
  56. S. Enayat and S. Banerjee, “Comparative antioxidant activity of extracts from leaves, bark and catkins of Salix aegyptiaca sp.,” Food Chemistry, vol. 116, no. 1, pp. 23–28, 2009. View at Publisher · View at Google Scholar · View at Scopus