Research Article

Pharmacological Basis for Traditional Use of the Lippia thymoides

Table 2

Content of total phenolics and antioxidant activity of crude extracts and fractions from leaves and stems of L. thymoides. Results expressed as mean ± SEM of three independent analyses.

SampleTotal phenolic content (mg/g GAE)DPPH scavenging (EC50, μg/mL)β-carotene bleaching (%)

TF115.5 ± 1.715.4 ± 1.69.3 ± 2.6
TFH15.5 ± 0.7333.7 ± 92.631.7 ± 3.2
TFD90.5 ± 1.022.1 ± 2.529.3 ± 6.0
TFA182.8 ± 2.45.7 ± 0.437.9 ± 4.1
TC85.5 ± 4.418.4 ± 2.43.7 ± 2.5
TCH14.5 ± 2.0585.1 ± 186.69.4 ± 1.0
TCD84.2 ± 6.114.5 ± 1.412.8 ± 0.4
TCA213.6 ± 6.14.5 ± 0.20
AcA5.1 ± 1.117.8 ± 5.6
BHT13.3 ± 1.0NT
BHA4.0 ± 0.6NT
QUENT36.2 ± 11.6
PIRNT4.9 ± 1.7

mg/g GAE, mg gallic acid equivalents per gram; EC50, concentration that caused 50% of the DPPH radical scavenging; NT, nontested; TF, crude methanolic extract from leaves; TFH, hexane fraction from TF; TFD, dichloromethane fraction from TF; TFA, ethyl acetate fraction from TF; TC, crude methanolic extract from stems; TCH, hexane fraction from TC; TCD, dichloromethane fraction from TC; TCA, ethyl acetate fraction from TC; AAc, ascorbic acid; BHT, butylated hydroxytoluene; BHA, butylated hydroxyanisole; QUE, quercetin; PYR, pyrogallol.