Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

The Herbal Compound “Diwu Yanggan” Modulates Liver Regeneration by Affecting the Hepatic Stem Cell Microenvironment in 2-Acetylaminofluorene/Partial Hepatectomy Rats

Figure 3

Effects of DWYG on 2-AAF/PH rat liver immunohistochemical indices. Normal Wistar rats (normal group), sham-operated rats (sham group), and 2-AAF/PH rats were orally administered vehicle (distilled water, vehicle group) or DWYG (DWYG group, 10 mg/kg). Paraffin sections were dewaxed and rehydrated and stained with the indicated primary antibodies. The mean integral optical density (IOD) was analyzed with Image Pro-Plus 6.0 software. (a) Representative images (left panel) of AFP immunohistochemistry and IOD (right panel). (b) IOD of CK19. (c) Representative images (left panel) of CD34 immunohistochemistry and IOD (right panel). (d–f) IOD of CD45 (d), Thy1.1, (e) and ALB (f). Data shown are means ± SD of 16 rats in each group. , versus vehicle group. Original magnification, 200x.

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