Research Article

Anti-Inflammatory Effects of Cumin Essential Oil by Blocking JNK, ERK, and NF-κB Signaling Pathways in LPS-Stimulated RAW 264.7 Cells

Figure 4

Effect of CuEO at different concentrations on the mRNA expression of LPS-induced IL-1β (a) and IL-6 (b) of RAW 264.7 cells. The cells (1 × 105 cells/well in a six-well plate) were pretreated with CuEO (0.001% to 0.01%) for 1 h and then stimulated with LPS (1 ug/mL). After 24 h incubation, total RNA was isolated and the mRNA expression was determined by real-time RT-PCR. Data represent the mean ± SD of three different experiments. and versus LPS-treated alone.
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