TY - JOUR
A2 - Wang, Yi-tao
AU - Hu, Zhigang
AU - Tu, Yuan
AU - Xia, Ye
AU - Cheng, Peipei
AU - Sun, Wei
AU - Shi, Yuhua
AU - Guo, Licheng
AU - He, Haibo
AU - Xiong, Chao
AU - Chen, Shilin
AU - Zhang, Xiuqiao
PY - 2015
DA - 2015/05/18
TI - Rapid Identification and Verification of Indirubin-Containing Medicinal Plants
SP - 484670
VL - 2015
AB - Indirubin, one of the key components of medicinal plants including Isatis tinctoria, Polygonum tinctorium, and Strobilanthes cusia, possesses great medicinal efficacy in the treatment of chronic myelocytic leukemia (CML). Due to misidentification and similar name, materials containing indirubin and their close relatives frequently fall prey to adulteration. In this study, we selected an internal transcribed spacer 2 (ITS2) for distinguishing these indirubin-containing species from five of their usual adulterants, after assessing identification efficiency of matK, rbcL, psbA-trnH, and ITS2 among these species. The results of genetic distances and neighbor-joining (NJ) phylogenetic tree indicated that ITS2 region is a powerful DNA barcode to accurately identify these indirubin-containing species and discriminate them from their adulterants. Additionally, high performance liquid chromatography (HPLC) was used to verify indirubin in different organs of the above species. The results showed that indirubin had been detected in the leaves of Is. tinctoria, P. tinctorium, S. cusia, and Indigo Naturalis (made from their mixture), but not in their roots, or in the leaves of their adulterants. Therefore, this study provides a novel and rapid method to identify and verify indirubin-containing medicinal plants for effective natural treatment of CML.
SN - 1741-427X
UR - https://doi.org/10.1155/2015/484670
DO - 10.1155/2015/484670
JF - Evidence-Based Complementary and Alternative Medicine
PB - Hindawi Publishing Corporation
KW -
ER -