Figure 3: Cell counts until 72 hours: overall increase in the cell count is observed 24 hours after culture (except for the control flask) which later decreases over 48 and 72 hours as the cells begin to aggregate, clump, and adhere to the bottom of the flask. The most significant increase was seen in 50 μg/mL from “0” hours to “24” hours compared to the other treated flasks. (Note that lymphocytes are resting cells which have been induced to multiply.)