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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 513978, 7 pages
http://dx.doi.org/10.1155/2015/513978
Research Article

Evaluation of Toxicity and Antimicrobial Activity of an Ethanolic Extract from Leaves of Morus alba L. (Moraceae)

1Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil
2Laboratório de Farmacologia e Cancerologia Experimental, Departamento de Antibióticos, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil
3Laboratório de Micologia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal da Paraíba, 58059-900 João Pessoa, PB, Brazil
4Departamento de Histologia e Embriologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, 50670-420 Recife, PE, Brazil

Received 4 May 2015; Accepted 28 June 2015

Academic Editor: Ilaria Lampronti

Copyright © 2015 Alisson Macário de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. World Health Organization, WHO Traditional Medicine Strategy: 2014–2023, World Health Organization, Geneva, Switzerland, 2013.
  2. K. O. Soetan and O. O. Aiyelaagbe, “The need for bioactivity-safety evaluation and conservation of medicinal plants—a review,” Journal of Medicinal Plants Research, vol. 3, no. 5, pp. 324–328, 2009. View at Google Scholar · View at Scopus
  3. D. G. I. Teles and M. M. Costa, “Estudo da ação antimicrobiana conjunta de extratos aquosos de Tansagem (Plantago major L., Plantaginaceae) e Romã (Punica granatum L., Punicaceae) e interferência dos mesmos na ação da amoxicilina in vitro,” Revista Brasileira de Plantas Medicinais, vol. 16, no. 2, pp. 323–328, 2014. View at Publisher · View at Google Scholar
  4. A. O. T. Ashafa and M. I. Kazeem, “Toxicopathological evaluation of hydroethanol extract of Dianthus basuticus in Wistar rats,” Evidence-Based Complementary and Alternative Medicine. In press.
  5. J. Zhishen, T. Mengcheng, and W. Jianming, “The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals,” Food Chemistry, vol. 64, no. 4, pp. 555–559, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Dugo, P. Donato, F. Cacciola, M. P. Germanò, A. Rapisarda, and L. Mondello, “Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system,” Journal of Separation Science, vol. 32, no. 21, pp. 3627–3634, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. S.-J. Piao, L.-X. Chen, N. Kang, and F. Qiu, “Simultaneous determination of five characteristic stilbene glycosides in root bark of Morus albus L. (Cortex Mori) using high-performance liquid chromatography,” Phytochemical Analysis, vol. 22, no. 3, pp. 230–235, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. S. H. Kim, N. J. Kim, J. S. Choi, and J. C. Park, “Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau,” Journal of the Korean Society of Food Science and Nutrition, vol. 22, pp. 68–72, 1993. View at Google Scholar
  9. T. Fukai, K. Kaitou, and S. Terada, “Antimicrobial activity of 2-arylbenzofurans from Morus species against methicillin-resistant Staphylococcus aureus,” Fitoterapia, vol. 76, no. 7-8, pp. 708–711, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. S.-J. Dai, Z.-B. Ma, Y. Wu, R.-Y. Chen, and D.-Q. Yu, “Guangsangons F-J, anti-oxidant and anti-inflammatory Diels-Alder type adducts, from Morus macroura Miq.,” Phytochemistry, vol. 65, no. 23, pp. 3135–3141, 2004. View at Publisher · View at Google Scholar · View at Scopus
  11. J.-H. Liang, Y.-W. Fu, Q.-Z. Zhang, D.-H. Xu, B. Wang, and D.-J. Lin, “Identification and effect of two flavonoids from root bark of Morus alba against Ichthyophthirius multifiliis in grass carp,” Journal of Agricultural and Food Chemistry, vol. 63, no. 5, pp. 1452–1459, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. A. Sheikhlar, A. R. Alimon, H. Daud et al., “White mulberry (Morus alba) foliage methanolic extract can alleviate Aeromonas hydrophila infection in African Catfish (Clarias gariepinus),” The Scientific World Journal, vol. 2014, Article ID 592709, 8 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  13. J. Qin, M. Fan, J. He et al., “New cytotoxic and anti-inflammatory compounds isolated from Morus alba L.,” Natural Product Research, 2015. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Yimam, Y. C. Lee, T. W. Kim et al., “UP3005, a botanical composition containing two standardized extracts of Uncaria gambir and Morus alba, improves pain sensitivity and cartilage degradations in monosodium iodoacetate­-induced rat OA disease model,” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 785638, 10 pages, 2015. View at Publisher · View at Google Scholar
  15. H. G. Kim, H. U. Jeong, G. Park, H. Kim, Y. Lim, and M. S. Oh, “Mori Folium and Mori Fructus mixture attenuates high-fat diet-induced cognitive deficits in mice,” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 379418, 8 pages, 2015. View at Publisher · View at Google Scholar
  16. B. Andallu and N. C. Varadacharyulu, “Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats,” Clinica Chimica Acta, vol. 338, no. 1-2, pp. 3–10, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. S.-H. Fang, Y.-C. Hou, and P.-D. L. Chao, “Pharmacokinetic and pharmacodynamic interactions of morin and cyclosporin,” Toxicology and Applied Pharmacology, vol. 205, no. 1, pp. 65–70, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. W. Wang, Y. Zu, Y. Fu, and T. Efferth, “In vitro antioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits,” The American Journal of Chinese Medicine, vol. 40, no. 2, pp. 349–356, 2012. View at Publisher · View at Google Scholar · View at Scopus
  19. J.-H. Lee, S. Y. Bae, M. Oh, K. H. Kim, and M. S. Chung, “Antiviral effects of mulberry (Morus alba) juice and its fractions on foodborne viral surrogates,” Foodborne Pathogens and Disease, vol. 11, no. 3, pp. 224–229, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. K. H. Seo, D. Y. Lee, R. H. Jeong et al., “Neuroprotective effect of prenylated arylbenzofuran and flavonoids from Morus alba fruits on glutamate­induced oxidative injury in HT22 hippocampal cells,” Journal of Medicinal Food, vol. 18, no. 4, pp. 403–408, 2015. View at Publisher · View at Google Scholar
  21. K. R. Markhan, Techniques of Flavonoids Identification, Academic Press, London, UK, 1982.
  22. H. Wagner and S. Bladt, Plant Drug Analysis: A Thin Layer Chromatography Atlas, Springer, New York, NY, USA, 2nd edition, 2001.
  23. M. Abreu, Triagem fitoquímica de plantas por cromatografia em camada delgada (CCD), Universidade de Ouro Preto, Ouro Preto, Brazil, 2000.
  24. B. N. Meyer, N. R. Ferrigni, J. E. Putnam, L. B. Jacobson, D. E. Nichols, and J. L. McLaughlin, “Brine shrimp: a convenient general bioassay for active plant constituents,” Planta Medica, vol. 45, no. 1, pp. 31–34, 1982. View at Publisher · View at Google Scholar · View at Scopus
  25. E. R. Almeida, Estudo da atividade antinociceptiva central do extrato hidroalcoólico das sementes de Dioclea grandiflora Mart ex. Benth (Fabaceae) e dois de seus constituintes quimícos: Dioclenol e Dioflorina [Ph.D. thesis], Universidade Federal da Paraíba, Paraíba, Brazil, 2002.
  26. Organization for Economic Cooperation and Development, OECD Guideline for Testing of Chemicals. Guideline 423: Acute Oral Toxicity-Acute Toxic Class Method, Organization for Economic Cooperation and Development, Paris, France, 2001.
  27. J. Kiernan, Histological and Histochemical Methods: Theory and Practice, Cold Spring Harbor Laboratory Press, Long Island, NY, USA, 4th edition, 2008.
  28. C. M. Mann and J. L. Markham, “A new method for determining the minimum inhibitory concentration of essential oils,” Journal of Applied Microbiology, vol. 84, no. 4, pp. 538–544, 1998. View at Publisher · View at Google Scholar · View at Scopus
  29. A. Sartoratto, A. L. M. Machado, C. Delarmelina, G. M. Figueira, M. C. T. Duarte, and V. L. G. Rehder, “Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil,” Brazilian Journal of Microbiology, vol. 35, no. 4, pp. 275–280, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. J. R. Naidu, R. Ismail, and S. Sasidharan, “Acute oral toxicity and brine shrimp lethality of methanol extract of Mentha Spicata L. (Lamiaceae),” Tropical Journal of Pharmaceutical Research, vol. 13, no. 1, pp. 101–107, 2014. View at Publisher · View at Google Scholar · View at Scopus
  31. A. S. Leite, A. F. Dantas, G. L. S. Oliveira et al., “Evaluation of toxic, cytotoxic, mutagenic, and antimutagenic activities of natural and technical cashew nut shell liquids using the Allium cepa and Artemia salina bioassays,” BioMed Research International, vol. 2015, Article ID 626835, 16 pages, 2015. View at Publisher · View at Google Scholar
  32. P. Nwankpa, E. N. Agomuo, G. C. Uloneme, J. N. Egwurugwu, Y. N. Omeh, and G. C. Nwakwuo, “Effect of Phyllanthus amarus leaf extract on alterations of haematological parameters in Salmonellae typhi infested wistar albino rats,” Scientific Research and Essays, vol. 9, no. 1, pp. 7–12, 2014. View at Publisher · View at Google Scholar
  33. R. C. Ekeanyanwu and O. U. Njoku, “Acute and subacute oral toxicity study on the flavonoid rich fraction of Monodora tenuifolia seed in albino rats,” Asian Pacific Journal of Tropical Biomedicine, vol. 4, no. 3, pp. 194–202, 2014. View at Publisher · View at Google Scholar · View at Scopus
  34. V. T. Motta, Bioquímica clínica para o laboratório: princípios e interpretações, MedBook, 5th edition, 2009.
  35. V. Kuete, Toxicological Survey of African Medicinal Plants, Elsevier, 1st edition, 2014.
  36. M. K. Gautam and R. K. Goel, “Toxicological study of Ocimum sanctum Linn leaves: hematological, biochemical, and histopathological studies,” Journal of Toxicology, vol. 2014, Article ID 135654, 9 pages, 2014. View at Publisher · View at Google Scholar · View at Scopus
  37. M. A. Z. Alferah, “Toxicity induced histological changes in selected organs of male (Wistar) rats by Lawsonia inermis leaf extract,” European Journal of Medicinal Plants, vol. 2, no. 2, pp. 151–158, 2012. View at Publisher · View at Google Scholar
  38. S. L. Born, A. M. Api, R. A. Ford, F. R. Lefever, and D. R. Hawkins, “Comparative metabolism and kinetics of coumarin in mice and rats,” Food and Chemical Toxicology, vol. 41, no. 2, pp. 247–258, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. P. A. Akah, C. C. Osigwe, and C. S. Nworu, “Reversal of coumarin-induced toxicity by the extracts and fractions of Ageratum conyzoides,” Asian Journal of Medical Sciences, vol. 2, no. 3, pp. 121–126, 2010. View at Google Scholar
  40. R. Jayaraj, U. Deb, A. S. B. Bhaskar, G. B. K. S. Prasad, and P. V. L. Rao, “Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice,” Environmental Toxicology, vol. 22, no. 5, pp. 472–479, 2007. View at Publisher · View at Google Scholar · View at Scopus
  41. Y. Miyasaki, J. D. Rabenstein, J. Rhea et al., “Isolation and characterization of antimicrobial compounds in plant extracts against multidrug-resistant Acinetobacter baumannii,” PLoS ONE, vol. 8, no. 4, Article ID e61594, 2013. View at Publisher · View at Google Scholar · View at Scopus