The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus

Figure 3

The effect of membrane-permeabilizing agents on the susceptibility of MRSA (ATCC 33591) to shikonin (SKN). Bacterial viability was determined by the absorbance at 600 nm after incubation for 24 h with SKN (3.9 μg/mL, 0.25 MIC value), 0.01% Tris (a), and 0.01% Triton X-100 (TX, (b)), or their combinations SKN + Tris and SKN + TX, respectively. The data are presented as the mean ± S.D. of three independent experiments. and compared to SKN alone. CON, untreated control MRSA.

(a)

(b)