Free Radical Scavenging Activity: Antiproliferative and Proteomics Analyses of the Differential Expression of Apoptotic Proteins in MCF-7 Cells Treated with Acetone Leaf Extract of Diospyros lycioides (Ebenaceae)

Table 2

A summary of the identification results from the spots excised from coomassie-stained 2D gels from proteins isolated from MCF-7 cells.

Spot	BLASTed; Pep. seq	NCBI; accession	MW; (kDa)	pI	Score	Identified protein
1	AAVPSGASTGIYEALELR	EAW90376	34	8.19	56.6 bits (126)	Enolase 3
2	IENHEGVRRFDEILEASDGIMVAR	AAH94767	58	7.58	81.2 bits (184)	PKM2
3	LISWYDNEFGYSNR	NP_001243728	34	8.26	54.9 bits (122)	GAPDH

Spot 1: enolase-3 (beta-muscle) isoform CRA_b (Homo sapiens).
Spot 2: pyruvate kinase-2, PKM2 (Homo sapiens).
Spot 3: glyceraldehyde-3-phosphate dehydrogenase isoform 2, GAPDH (Homo sapiens).