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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 587383, 7 pages
http://dx.doi.org/10.1155/2015/587383
Research Article

Inhibitory Properties of Aqueous Ethanol Extracts of Propolis on Alpha-Glucosidase

1Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China
2National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China
3Department of Food Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2

Received 4 September 2014; Revised 15 December 2014; Accepted 18 December 2014

Academic Editor: Flávia R. F. do Nascimento

Copyright © 2015 Hongcheng Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of the present study was to evaluate the inhibitory properties of various extracts of propolis on alpha-glucosidase from baker’s yeast and mammalian intestine. Inhibitory activities of aqueous ethanol extracts of propolis were determined by using 4-nitrophenyl-D-glucopyranoside, sucrose and maltose as substrates, and acarbose as a positive reference. All extracts were significantly effective in inhibiting α-glucosidase from baker’s yeast and rat intestinal sucrase in comparison with acarbose (). The 75% ethanol extracts of propolis (75% EEP) showed the highest inhibitory effect on α-glucosidase and sucrase and were a noncompetitive inhibition mode. 50% EEP, 95%, EEP and 100% EEP exhibited a mixed inhibition mode, while water extracts of propolis (WEP) and 25% EEP demonstrated a competitive inhibition mode. Furthermore, WEP presented the highest inhibitory activity against maltase. These results suggest that aqueous ethanol extracts of propolis may be used as nutraceuticals for the regulation of postprandial hyperglycemia.