Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes

Figure 1

Effects of hinokitiol on cell viability and interferon gamma (IFN-γ) secretion in ConA-activated T lymphocytes. Cells were treated with hinokitiol (1–5 μM) in the absence or presence of ConA (10 μg/mL) for 24 or 48 h. (a, b) Cell viability was determined using a MTT assay (). (c) The level of IFN-γ was measured by an ELISA assay (). Data (b, c) are presented as the mean ± SEM ( and compared with solvent control (DMSO); ^# and ^## compared with the ConA-treated group).

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