Inhibitory Effects of Chemical Compounds Isolated from the Rhizome of Smilax glabra on Nitric Oxide and Tumor Necrosis Factor-α Production in Lipopolysaccharide-Induced RAW264.7 Cell

Figure 1

Effects of ethanol extract of S. glabra (ESG) and its four subfractions (ESG-1, ESG-2, ESG-3, and ESG-4) on RAW264.7 cells viability and LPS-induced NO production. Cells viability (a) was tested by MTT assay; higher value of optical density at 490 nm means higher cell viability. Nitrite accumulated in cell culture supernatants was determined by Griess assay as an index for NO release (b). Cells were treated with LPS (1 μg/mL) for 24 h in the absence or presence of samples (1.6, 8.0, 40, and 200.0 μg/mL). The data were presented as mean ± S.D. (). Dexamethasone (DMS) was employed as a positive control. # and ###, respectively, mean and compared with control group. , , and , respectively, mean , , and compared with group treated with LPS alone.

(a)

(b)