AP-1-Targeting Anti-Inflammatory Activity of the Methanolic Extract of Persicaria chinensis

Figure 3

Effect of Pc-ME on the activation of proinflammatory cytokines and transcriptional regulation. (a) Levels of c-Fos, c-Jun, and lamin A/C in nuclear fractions were determined by immunoblot analysis in RAW264.7 cells. (b) Phospho- or total protein levels of c-Fos and β-actin in cell lysates were determined by immunoblot analysis in U937 cells. (c) mRNA levels of TNF-α, IL-1β, IL-6, and GAPDH were determined by semiquantitative RT-PCR in RAW264.7 cells. (d–f) mRNA levels of TNF-α, IL-1β, and IL-6 were determined by real-time RT-PCR in U937 cells.

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