Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 1

Research Article

A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

Figure 1

Gintonin inhibited LPS mediated NO release without any cytotoxicity. RAW 264.7 cells were seeded in 96-well plate for 18 h and then preincubated with gintonin (6.25 μg/mL–50 μg/mL) for 30 min and then stimulated with LPS (0.1 μg/mL) for 18 h. The cell supernatant was then transferred to a 96-well plate and mixed with equal amounts of Griess reagent, and then NO production was measured at 540 nm (a). Effects of gintonin on cell viability were measured by MTT assay and absorbance was measured at 560 nm (b). Values in bar graph are mean ± SEM of at least 4 independent experiments. compared to LPS only.