Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 4

Research Article

A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

Figure 4

Attenuation of NF-κB pathway by gintonin. RAW 264.7 cells were pretreated with gintonin in the indicated concentrations (6.25 μg/mL–50 μg/mL) and then stimulated with or without LPS (0.1 μg/mL) for 18 h. Nuclear and cytosolic proteins were extracted using NE-PER extraction kit. Proteins were run on SDS-PAGE and treated with primary antibodies overnight followed by incubation with secondary antibodies for 2 h. The membranes were analyzed by ECL chemiluminescence systems. Data are mean ± SD (); compared with LPS only. (a) Phosphorylation of IRAK-1 compared to β-actin. (b) Phosphorylation of P-TAK-1 compared to total TAK-1. (c) Phosphorylation of IKK α/β compared to β-actin. (d) Phosphorylation of P-IκBα compared to total IκBα. (e) Phosphorylation of NF-κB compared to β-actin.