Orostachys japonicus Inhibits Expression of the TLR4, NOD2, iNOS, and COX-2 Genes in LPS-Stimulated Human PMA-Differentiated THP-1 Cells by Inhibiting NF-κB and MAPK Activation

Figure 5

Effects of O. japonicus on LPS-induced NF-κB p65 phosphorylation and IκBα degradation in THP-1 cells. Cells were pretreated for 20 h with various concentrations of O. japonicus (10 or 25 μg/mL) before exposure to LPS (1 μg/mL) for 4 h, and NF-κB p65 and IκBα protein levels were determined using immunoblotting. Densitometric analyses were presented as the relative ratios of NF-κB p65 (a) or p-NF-κB p65 (c) and IκBα (b) or p-IκBα (d) to β-actin. The data represent the means ± SD of three independent samples. compared with LPS stimulation alone.

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