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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 687690, 9 pages
http://dx.doi.org/10.1155/2015/687690
Research Article

Effects of Soothing Liver and Invigorating Spleen Recipes on the IKK-NF-B Signaling Pathway in Kupffer Cells of Nonalcoholic Steatohepatitis Rats

Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China

Received 16 February 2015; Revised 19 May 2015; Accepted 31 May 2015

Academic Editor: Bashar Saad

Copyright © 2015 Xiang-Wen Gong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigates the effect of soothing liver and invigorating spleen recipes on steatohepatitis examining the IKKβ-NF-κB signaling pathway in KCs of NASH rats. SD male rats were randomly divided into 8 groups, and the NASH model was induced by a high-fat diet (HFD). After 26 weeks, liver tissue was examined in H&E stained sections and liver function was monitored biochemically. KCs were isolated by Seglen’s method, with some modifications. The mRNA and protein expression of the IKKβ-NF-κB signaling pathway components was examined by quantitative PCR and Western blotting. The results show that the high-fat diet induced NASH in the rats, and the soothing liver recipe and invigorating spleen recipe decreased the levels of TNF-α, IL-1, and IL-6 in KCs, as well as inhibiting the mRNA and protein expression of the IKKβ-NF-κB signaling pathway components. In conclusion, the experiment indicated the importance of the IKKβ-NF-κB signaling pathway in KCs for the anti-inflammatory effects of the soothing liver and invigorating spleen recipes.