Curcumin Protects Neurons from Glutamate-Induced Excitotoxicity by Membrane Anchored AKAP79-PKA Interaction Network
Curcumin enhanced the interaction between AKAP79 and PKA at the plasma membrane. (a) The change of AKAP79-PKA interaction after exposure to curcumin with or without glutamate exposure. (b) Western blotting bands were quantified using Image J software. (c) The colocalization changes between AKAP79 and PKA in membrane. The red fluorescent indicated the AKAP79 staining and the green fluorescent showed the PKA staining, while nucleus stained by Hoechst 33342 emitted blue fluorescent. The red fluorescent and green fluorescent merged into yellow staining which suggested the overlap between AKAP79 and PKA. Scale bar, 50 μm. The colocalization of AKAP79 and PKA was quantified using Image J software. and versus control values. ().
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