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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 734903, 9 pages
http://dx.doi.org/10.1155/2015/734903
Research Article

Cardioprotective Potential of Polyphenolic Rich Green Combination in Catecholamine Induced Myocardial Necrosis in Rabbits

1Department of Chemistry, University of Agriculture, Faisalabad 38000, Pakistan
2Department of Biochemistry, University of Agriculture, Faisalabad 38000, Pakistan
3Department of Pathology, University of Agriculture, Faisalabad 38000, Pakistan
4Department of Entomology, University of Agriculture, Faisalabad 38000, Pakistan

Received 5 February 2015; Revised 13 May 2015; Accepted 21 May 2015

Academic Editor: Joen-Rong Sheu

Copyright © 2015 Fatiqa Zafar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was designed to develop safer, effective, and viable cardioprotective herbal combination to control oxidative stress related cardiac ailments as new alternatives to synthetic drugs. The synergetic cardioprotective potential of herbal combination of four plants T. arjuna (T.A.), P. nigrum (P.N), C. grandiflorus (C), and C. oxyacantha (Cr) was assessed through curative and preventive mode of treatment. In preventive mode of treatment, the cardiac injury was induced with synthetic catecholamine (salbutamol) to pretreated rabbits with the proposed herbal combination for three weeks. In curative mode of treatment, cardiotoxicity/oxidative stress was induced in rabbits with salbutamol prior to treating them with plant mixture. Cardiac marker enzymes, lipids profile, and antioxidant enzymes as biomarker of cardiotoxicity were determined in experimental animals. Rabbits administrated with mere salbutamol showed a significant increase in cardiac marker enzymes and lipid profile and decrease in antioxidant enzymes as compared to normal control indicating cardiotoxicity and myocardial cell necrosis. However, pre- and postadministration of plant mixture appreciably restored the levels of all biomarkers. Histopathological examination confirmed that the said combination was safer cardioprotective product.