Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

Antiproliferative and Apoptosis-Inducing Activities of 4-Isopropyl-2,6-bis(1-phenylethyl)phenol Isolated from Butanol Fraction of Cordyceps bassiana

Figure 3

Apoptosis-inducing effects of KTH-13 in C6 glioma cells. (a) C6 glioma cells (5 × 106 cells/mL) were incubated with KTH-13 or staurosporine (STS) for 24 h. After preparing DNA extracts, DNA laddering patterns were evaluated by agarose gel electrophoresis. (b) Cell apoptosis was determined by Annexin V-FITC and PI double-staining analysis. The KTH-13- or STS-treated apoptotic cells with Annexin V and PI treatment were analyzed by flow cytometry. Images from three experiments are shown. (c) Morphological changes of C6 glioma (A) and MDA-MB-231 (B) cells induced by KTH-13 were observed by microscopic analysis. All of the data were obtained from one of three independent experiments.

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